ASHUTOSH CHILKOTI
Associate Director, CBIMMS
Professor, Department of Biomedical Engineering

Contact Information
Department of Biomedical Engineering
3381 CIEMAS, Box 90281, Duke University
Durham, NC 27708
(PH) 919-660-5373
(FX) 919-684-4488
chilkoti@duke.edu

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Education

	PhD	Surface Chemistry of Organic Plasma-Deposited Films Created from Oxygen-Containing Precursors, Chemical Engineering, University of Washington, Seattle, WA, 1991
	B.Tech.	Chemical Engineering, Indian Institute of Technology, Delhi, India, 1985

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Experience

	2003-present	Associate Professor, Department of Biomedical Engineering, Duke University
	2002-present	Associate Director, Center for Biologically Inspired Materials and Material Systems
	1996-2002	Assistant Professor, Department of Biomedical Engineering, Duke University
	1992-1995	Research Associate, Center for Bioengineering, University of Washington
	1991-1992	Postdoctoral Fellow, Department of Chemical Engineering, University of Washington

 

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Selected Publications

1. N Nath and A Chilkoti. A colorimetric colloidal gold sensor to interrogate biomolecular interactions in real-time on a surface, Anal. Chem. accelerated article, 74: 504-509 (2002).
2. N Nath and A Chilkoti. Interfacial phase transition of an environmentally responsive elastin biopolymer adsorbed on a self-assembled monolayer on gold studied by colloidal surface plasmon resonance, J. Am. Chem Soc., 123: 8197-8202 (2001).
3. W Frey, CK Woods, and A Chilkoti. Ultraflat nanosphere lithography: A new method to fabricate flat nanostructures, Adv. Mater. 12: 1515-1519 (2000).
4. J Hyun, SJ Ahn, W Lee, A Chilkoti, and S Zauscher. Molecular recognition mediated fabrication of protein nanostructures by dip-pen lithography, Nanoletters, 2: 1203-1207 (2002).
5. J Hyun and A Chilkoti. Micropatterning biological molecules on a polymer surface using elastomeric microwells, J. Am. Chem Soc., 123: 6943-6944 (2001).
6. J Hyun and A Chilkoti. Surface-initiated free radical polymerization of polystyrene micropatterns on a self-assembled monolayer on gold, Macromolecules, 16: 5644-5652 (2001).
7. A Belu, Z-P Yang, R Aslami, and A Chilkoti. Enhanced TOF-SIMS imaging of a micropatterned protein by 15N labeling, Anal. Chem., accelerated article, 73: 143-150 (2001).
8. Z-P Yang and A Chilkoti. Microstamping of a biological ligand onto an activated polymer surface, Adv. Mater., 12: 413-417 (2000).
9. DE Meyer and A Chilkoti. Purification of recombinant proteins by fusion with thermally responsive polypeptides, Nature Biotechnology 17: 1112-1115 (1999).
10. DE Meyer, GH Kong, MW Dewhirst, M Zalutsky and A Chilkoti. Targeting a genetically engineered elastin-like polypeptide to solid tumors by local hyperthermia. Cancer Res. 61: 1548-1554 (2001).D.
    

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Fellowships and Awards

	2002	3M Nontenured Faculty Award
	1998	NSF CAREER Award
	1989	Student Paper Prize, American Vacuum Society, Pacific Northwest Chapter Symposium
	1978-1985	National Merit Scholarship, National Council of Educational Research and Training, India

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Short Research Interest Descriptor

The overall goal of my research is the design, characterization, and application of engineered biomolecules with a primary focus on control of their physico-chemical properties, and their spatial and temporal organization at molecular dimensions.

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Research Interest

In the first area of research, Biomolecular Engineering, we synthesize “switchable” biopolymers and fusion proteins (e.g., biomolecules that exhibit reversible solubility and reversible binding to surfaces) in response to an external trigger (e.g., heat, light, pH) and exploit their switchable behavior in biotechnology (protein purification and proteomics), medicine (clinical assays and drug delivery) and materials science (e.g., actuators and tissue engineering scaffolds).

In a second, related area of Biomolecular Surface Science, we engineer the organization of biomolecules at molecular dimensions. This research is motivated by the rationale that the organization of biomolecules at the nanometer to micron length scale is useful for numerous applications that include the design of bioactive materials, and the fabrication of microfluidic devices for bionalytical applications and cell-based sensors. In one approach, we have developed “top-down” fabrication methods that involve printing biomolecules on diverse surfaces at micrometer length scales (section A.2.1); this work is now being “reduced” down to the nanometer length scales by variants of dip-pen lithography that are compatible with biomolecules. We have also developed “bottom up” fabrication methods in which the spatial organization of a biomolecule in 2-D is dictated by the thermodynamics of the system.

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